Capture of genomic DNA on glass microscope slides

Glass slides to DNA microarrays

on genomics, became aware of the potential commercial, technical, and social importance of the human genome project during the late 1990s. The human genome project was formally initiated in 19901 and was expected to last 15 years. It had the major goals of identifying all the genes in human DNA, determining the sequences of those genes, and storing the information in public databases. However, ...

Extraction and genotyping of Cryptosporidium parvum DNA from fecal smears on glass slides stained conventionally for direct microscope examination.

A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infectious agents.

Dendrimeric coating of glass slides for sensitive DNA microarrays analysis.

Successful use and reliability of microarray technology is highly dependent on several factors, including surface chemistry parameters and accessibility of cDNA targets to the DNA probes fixed onto the surface. Here, we show that functionalisation of glass slides with homemade dendrimers allow production of more sensitive and reliable DNA microarrays. The dendrimers are nanometric structures of...

Preparation of DNA and protein micro arrays on glass slides coated with an agarose film.

A thin layered agarose film on microscope slides provides a versatile support for the preparation of arrayed molecular libraries. An activation step leading to the formation of aldehyde groups in the agarose creates reactive sites that allow covalent immobilization of molecules containing amino groups. Arrays of oligonucleotides and PCR products were prepared by tip printing. After hybridizatio...

Cryptosporidium Genotyping Method for Regulatory Microscope Slides